Composite

Part:BBa_K1778003:Design

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-11)


RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When using the part in Pichia pastoris, we need to consider whether the protein is stable in yeast cells and whether the formation of its conformation is effective to identify specific promoter, which requires the support of experiments and the paper.


Source

rtTA is from the commercial plasmid pTet-on,flag tags is commonly used detective tags.

References